Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multilayered regulation of chloroplast development by single B-BOX CONSTANS-LIKE and GOLDEN2-LIKE proteins in Arabidopsis.

Plant physiology·2026
Same author

JAK Inhibitor-Induced Improvement of IgA Nephropathy in a Patient With Polycythemia Vera: A Case Report.

Kidney medicine·2026
Same author

Chromid-like secondary replicons as predicted key sites of biosynthetic gene clusters in <i>Ktedonobacteria</i>.

mSystems·2026
Same author

Multi-omics dissection of steviol glycoside synthesis reveals haplotype-linked specialization of UGT76G genes in Stevia rebaudiana.

The New phytologist·2026
Same author

[HLA-haploidentical hematopoietic stem cell transplantation with post-transplant cyclophosphamide in patients with myelofibrosis].

[Rinsho ketsueki] The Japanese journal of clinical hematology·2026
Same author

Chloroplast stress caused by maltose hyperaccumulation activates chlorophagy via the core autophagy machinery.

Plant physiology·2026

Related Experiment Video

Updated: Jun 26, 2025

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

29.9K

High-throughput mechanical phenotyping and transcriptomics of single cells.

Akifumi Shiomi1,2, Taikopaul Kaneko1, Kaori Nishikawa1

  • 1Cluster for Pioneering Research, RIKEN, Saitama, Japan.

Nature Communications
|May 17, 2024
PubMed
Summary

Researchers developed ELASTomics, a new method combining cell surface tension measurement with transcriptomics. This technique allows detailed study of how mechanical properties and gene expression interact in single cells.

More Related Videos

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

17.6K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K

Related Experiment Videos

Last Updated: Jun 26, 2025

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

29.9K
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

17.6K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K

Area of Science:

  • Cell biology
  • Biophysics
  • Genomics

Background:

  • Understanding cellular mechanical properties at single-cell resolution is challenging.
  • Existing methods lack the ability to combine mechanophenotyping with unbiased transcriptional screening.

Purpose of the Study:

  • To introduce ELASTomics, a novel method for simultaneous measurement of cell surface tension and gene expression.
  • To enable joint analysis of cell mechanics and transcriptional regulation at single-cell resolution.

Main Methods:

  • ELASTomics utilizes electroporation to import oligonucleotide-labelled macromolecules into cells.
  • Macromolecules assess cell surface tension, and their abundance is determined by sequencing.
  • The method integrates with existing single-cell sequencing technologies.

Main Results:

  • ELASTomics successfully validated in diverse cancer cell lines, accurately identifying cell types and measuring surface tension.
  • The study explored relationships between cell surface tension, proteins, and transcripts during hematopoietic progenitor cell differentiation.
  • RRAD was identified as a regulator of cell surface tension in senescent TIG-1 cells.

Conclusions:

  • ELASTomics offers a powerful tool for profiling single-cell mechanical and molecular phenotypes.
  • The method facilitates dissection of the interplay between cell mechanics and gene expression in various biological contexts.
  • This approach opens new avenues for research in cell biology and biophysics.