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Mouse Wound Models and Preparation of Single-Cell Suspensions
Published on: September 27, 2024
541
Plasma-Based Scaffold Containing Bone-Marrow Mononuclear Cells Promotes Wound Healing in a Mouse Model of Pressure
Maria Alvarez-Viejo1,2,3, Luis Romero-Rosal4, Marcos Perez-Basterrechea1,2
1Unit of Cell Therapy and Regenerative Medicine, Department of Hematology and Hemotherapy, Central University Hospital of Asturias, Oviedo, Spain.
Cell Transplantation
|May 18, 2024
Summary
Bone marrow mononuclear cells (BM-MNCs) in a plasma scaffold show potential for treating pressure injuries by reducing inflammation and promoting healing in a mouse model. Further research is needed to confirm wound closure efficacy.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Wound Healing Research
Background:
- Pressure injuries (ulcers) pose significant clinical and economic challenges.
- Current treatments, including surgery, have limitations and potential complications.
- Advanced therapies offer promising new avenues for pressure injury management.
Purpose of the Study:
- To investigate the efficacy of bone marrow mononuclear cells (BM-MNCs) within a plasma scaffold for pressure injury treatment.
- To evaluate the impact of this advanced therapy on wound healing, inflammation, and cellular integration in a preclinical model.
Main Methods:
- Pressure ulcers were induced in mice and treated with either a plasma scaffold alone or a plasma scaffold with BM-MNCs.
- Biometric assessment, bioluminescence imaging, and tomography were used for in-vivo analysis.
- Histological examination and RT-PCR array studies were performed on skin biopsies to assess wound healing and gene expression.
Main Results:
- While not statistically significant, wound closure was numerically higher in treatment groups compared to controls.
- Immunohistochemistry confirmed the presence of donor BM-MNCs in treated wounds.
- The Plasma + BM-MNC group exhibited reduced inflammation (PET/CT) and complete morphometabolic response by Day 14, supported by histological data.
- Gene expression analysis revealed significant differences, particularly between treatment groups at Day 14.
Conclusions:
- Plasma scaffolds containing BM-MNCs demonstrate a capacity to modulate the inflammatory response and support tissue regeneration in pressure injuries.
- The observed reduction in inflammation and positive histological changes suggest therapeutic potential for BM-MNCs in wound healing.
- Further investigation is warranted to optimize this advanced therapy for clinical application in pressure injury treatment.
