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Related Experiment Video

Updated: Jun 26, 2025

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LncRNA SH3BP5-AS1 promotes hepatocellular carcinoma progression by sponging miR-6838-5p and activation of PTPN4.

Xinyang Zhao1, Xinfeng Zhu1, Chaowen Xiao1

  • 1Department of Hepatobiliary Surgery, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, Hubei, China.

Aging
|May 18, 2024
PubMed
Summary
This summary is machine-generated.

Long noncoding RNA SH3BP5-AS1 promotes hepatocellular carcinoma (HCC) growth by regulating miR-6838-5p and PTPN4. This study identifies SH3BP5-AS1 as a novel oncogenic long noncoding RNA (lncRNA) in HCC development.

Keywords:
HIF-1αPTPN4SH3BP5-AS1hepatocellular carcinomamiR-6838-5p

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (LncRNAs) play critical roles in hepatocellular carcinoma (HCC) development.
  • Identifying novel lncRNAs involved in carcinogenesis is crucial for understanding HCC.
  • LncRNA SH3BP5-AS1's specific function in HCC remains largely undetermined.

Purpose of the Study:

  • To investigate the role and mechanism of LncRNA SH3BP5-AS1 in the carcinogenesis of HCC.
  • To elucidate the regulatory pathway involving SH3BP5-AS1, miR-6838-5p, and PTPN4 in HCC.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect expression levels of SH3BP5-AS1, miR-6838-5p, and PTPN4.
  • Cellular functionality assays, including proliferation, migration, and invasion assays, were performed.
  • Glycolysis assays and cell-derived xenograft models were utilized to assess functional impacts.

Main Results:

  • High expression of LncRNA SH3BP5-AS1 correlated with advanced tumor stage in HCC.
  • SH3BP5-AS1 overexpression enhanced HCC cell invasion, migration, and proliferation.
  • SH3BP5-AS1 promoted glycolysis by sponging miR-6838-5p and activating PTPN4, a direct target of miR-6838-5p.
  • HIF-1α was identified as a transcriptional regulator of SH3BP5-AS1.

Conclusions:

  • LncRNA SH3BP5-AS1 acts as an oncogenic lncRNA in HCC progression.
  • SH3BP5-AS1 exerts its oncogenic effects by regulating the miR-6838-5p/PTPN4 axis.
  • The findings highlight SH3BP5-AS1 as a potential therapeutic target for HCC.