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M-F noncoding region sequences of H1 genotype measles virus provide higher resolution for virus transmission tracing

Jinhua Song1, Zhen Zhu1, Huiling Wang1

  • 1WHO Western Pacific Regional Measles/Rubella Reference Laboratory, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Virology
|May 18, 2024
PubMed

Insights

The noncoding region between measles virus matrix and fusion protein genes (M-F NCR) enhances molecular surveillance. This extended sequence window improves tracking of measles virus transmission chains, complementing existing methods.

Area of Science:

  • Virology
  • Epidemiology
  • Molecular Biology

Background:

  • Measles elimination efforts necessitate robust molecular surveillance tools.
  • Traditional molecular markers may lack sufficient resolution for detailed transmission chain analysis.

Purpose of the Study:

  • To evaluate the molecular resolution of the M-F NCR sequence for measles virus surveillance.
  • To compare the utility of M-F NCR with the N450 target in characterizing measles virus strains.

Main Methods:

  • Phylogenetic analysis of 192 genotype H1 measles virus strains from China (2011-2018).
  • Comparison of molecular resolution between N450 and M-F NCR targets.

Main Results:

  • Both N450 and M-F NCR targets confirmed outbreak links.
  • M-F NCR provided higher molecular resolution, differentiating strains with identical N450.
  • M-F NCR distinguished transmission chains and improved spatial-temporal consistency.

Conclusions:

  • The M-F NCR is a valuable target for enhancing molecular characterization of measles virus.
  • M-F NCR complements N450 data for detailed tracking of virus transmission chains during elimination efforts.
Keywords:
M-F NCRMeasles eliminationMeasles virus

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