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Updated: Jun 26, 2025

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
Published on: November 29, 2014
Coupling of surface plasmon resonance and mass spectrometry for molecular interaction studies in drug discovery
Yuandi Zhao1, Darya Hadavi1, Ingrid Dijkgraaf2
1Maastricht Multimodal Molecular Imaging (M4i) Institute, Maastricht University, Maastricht, the Netherlands.
Abstract:
Various analytical technologies have been developed for the study of target-ligand interactions. The combination of these technologies gives pivotal information on the binding mechanism, kinetics, affinity, residence time, and changes in molecular structures. Mass spectrometry (MS) offers structural information, enabling the identification and quantification of target-ligand interactions. Surface plasmon resonance (SPR) provides kinetic information on target-ligand interaction in real time. The coupling of MS and SPR complements each other in the studies of target-ligand interactions. Over the last two decades, the capabilities and added values of SPR-MS have been reported. This review summarizes and highlights the benefits, applications, and potential for further research of the SPR-MS approach.
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