Related Experiment Video

Updated: Jun 26, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.0K

(G)Patching up mis-splicing in cancer

Maciej Cieśla1, Cristian Bellodi2

  • 1IMol Polish Academy of Sciences, Warsaw, Poland.

Trends in Biochemical Sciences
|May 18, 2024
PubMed

Insights

The G-patch motif protein GPATCH8 is crucial in cancer-driving mutations of the splicing factor SF3B1. Targeting GPATCH8 offers new therapeutic strategies for SF3B1 mutant cancers and splicing-related diseases.

Area of Science:

  • Molecular Biology
  • Cancer Genetics
  • RNA Splicing

Background:

  • Mutations in the splicing factor SF3B1 are common in various cancers.
  • Aberrant RNA splicing contributes to cancer development and progression.

Purpose of the Study:

  • To investigate the role of GPATCH8 in SF3B1-mutant cancers.
  • To explore GPATCH8 as a potential therapeutic target.

Main Methods:

  • Utilized molecular biology techniques to study protein interactions.
  • Analyzed splicing patterns in cancer cell lines with SF3B1 mutations.

Main Results:

  • GPATCH8 cooperates with mutant SF3B1, leading to aberrant splicing.
  • GPATCH8 is essential for the mis-splicing phenotype in SF3B1-mutant cancers.

Conclusions:

  • GPATCH8 plays a critical role in the pathogenesis of SF3B1-mutant cancers.
  • Targeting GPATCH8 presents a promising therapeutic avenue for these cancers and other splicing-related disorders.
Keywords:
DHX15G-patch domainGPATCH8SF3B1SUGP1splicing

More Related Videos

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.7K
Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer09:38

Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer

Published on: March 28, 2018

12.3K

Related Concept Videos

Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.6K
Mismatch Repair01:36

Mismatch Repair

Overview
40.1K
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
37.0K
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
22.3K