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Updated: Jun 30, 2026
Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue
Published on: September 10, 2019
NEUT-RI, a surrogate marker of NETosis is lower in patients with strong IgM antiphospholipid antibodies
Sylvie Bouvier1,2,3, Mathieu Fortier4, Eva Nouvellon4,5
1Department of Hematology, Nîmes University Hospital, Place du Professeur Robert Debré, Nîmes, 30029, France. sylvie.bouvier@chu-nimes.fr.
Background:
Antiphospholipid antibody syndrome (APS) is an acquired autoimmune disorder characterized by recurrent venous or arterial thrombosis and/or pregnancy complications. Recently, thrombotic APS was linked to increased neutrophil extracellular traps (NET) formation, suggesting an association between NETs and the severity of APS-related thrombosis.
Methods:
We performed a retrospective study on patients tested for presence of antiphospholipid antibodies (990 negative and 374 positive) to evaluate the association between the neutrophil activation state, estimated by the neutrophil reactive index (NEU-RI), a parameter routinely available from some haematology analysers, and antiphospholipid antibodies.
Results:
We do not observe a difference in NEU-RI values between positive and negative patients globally. However, interestingly, we highlight an association between high titers of IgM and low NEU-RI values indicating a lower neutrophil activation.
Conclusion:
Our data are in line with the recent questioning about the putative clinical consistency of positive solid-phase aPL IgM.
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