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Related Experiment Videos

Granular natural-killer cells develop into mucus-secreting cells.

H Ginsburg, T Yehuda-Cohen, R Coleman

    Immunology Letters
    |January 1, 1985
    PubMed
    Summary

    Natural killer (NK) cells in nude mice, when cultured with embryonic cells, produce and secrete mucus. This process involves granule erosion and vesicle budding, linked to mesenchymal cell growth and epithelial development.

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    Area of Science:

    • Immunology
    • Developmental Biology
    • Cell Biology

    Background:

    • Nude mice are commonly used in immunological research due to their deficient T-cell immunity.
    • Natural killer (NK) cells are crucial components of the innate immune system.
    • Embryonic cell cultures provide a model for studying developmental processes.

    Purpose of the Study:

    • To investigate the differentiation and function of granular natural-killer cells in vitro.
    • To explore the interaction between NK cells and embryonic cells.
    • To identify the secreted products of NK cells under specific culture conditions.

    Main Methods:

    • Culture of lymph-node cells from nude mice.
    • Stimulation with rat T-cell growth factor.

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  • Co-culture with X-irradiated embryonic mouse cell monolayers.
  • Analysis of secreted glycoproteins and cellular processes.

    • Main Results:

    • Colonies of granular natural-killer cells developed in vitro.
    • These cells synthesized and secreted a sulphated glycoprotein identified as mucus.
    • Mucus accumulation occurred via granule erosion and vesicle budding.
    • Mucous synthesis correlated with mesenchymal cell growth and epithelial morphogenesis induction.

    Conclusions:

    • Granular natural-killer cells can be induced to synthesize and secrete mucus.
    • The process involves specific cellular mechanisms like granule erosion and vesicle budding.
    • This phenomenon is linked to the developmental potential of co-cultured embryonic mesenchymal cells.