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Effect of baicalin on eradicating biofilms of bovine milk derived Acinetobacter lwoffii

Chengjun Ma1,2,3, Cui Mei1,2,3, JingJing Liu1,2,3

  • 1College of Veterinary Medicine, Southwest University, Chongqing, 402460, China.

BMC Veterinary Research
|May 19, 2024
PubMed
Abstract

Insights

Baicalin effectively eradicates Acinetobacter lwoffii biofilms in dairy cows by impacting bacterial genes and trehalose levels. This natural compound shows promise in combating drug-resistant bacterial infections.

Area of Science:

  • Microbiology
  • Bacteriology
  • Drug Discovery

Background:

  • Acinetobacter lwoffii (A.lwoffii) is a zoonotic pathogen causing severe infections like meningitis and pneumonia.
  • Increasing A.lwoffii infections, particularly in livestock, are linked to resilient biofilms, posing a significant public health threat.
  • Eradicating preformed biofilms is crucial for controlling A.lwoffii and preventing the spread of antimicrobial resistance.

Purpose of the Study:

  • To investigate the efficacy of baicalin in eradicating preformed A.lwoffii biofilms in dairy cows.
  • To elucidate the underlying molecular mechanisms by which baicalin exerts its antibiofilm effects.

Main Methods:

  • Treatment of A.lwoffii biofilms with varying concentrations of baicalin.
  • Quantification of viable bacteria and biofilm biomass using colony-forming units and fluorescence intensity.
  • Transcriptomic analysis to identify differentially expressed genes.
  • Molecular docking to predict baicalin-protein interactions.
  • Measurement of trehalose content in biofilms.

Main Results:

  • Baicalin at 4 times the minimum inhibitory concentration (MIC) significantly eradicated preformed A.lwoffii biofilms, reducing viable bacteria by 0.67 Log10CFU/mL.
  • A 67.0% reduction in total fluorescence intensity indicated significant antibiofilm activity.
  • Transcriptomic analysis revealed 833 differentially expressed genes involved in oxidative phosphorylation, biofilm regulation, and trehalose synthesis.
  • Molecular docking identified 11 potential target proteins for baicalin, and trehalose content decreased post-treatment.

Conclusions:

  • Baicalin demonstrates potent antibiofilm activity against A.lwoffii.
  • The biofilm eradication by baicalin is likely mediated by its effects on oxidative phosphorylation and two-component systems (TCSs).
  • A significant decrease in trehalose content correlates with baicalin-induced biofilm eradication, suggesting its role in the mechanism.
Keywords:
Acinetobacter lwoffiiBaicalinBiofilmEradicationTrehalose

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