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Related Concept Videos

CRISPR01:59

CRISPR

50.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Experimental RNAi02:15

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Related Experiment Video

Updated: Jun 26, 2025

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
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Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

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Programmable RNA targeting with CRISPR-Cas13.

Peiguo Shi1, Xuebing Wu1

  • 1Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA.

RNA Biology
|May 20, 2024
PubMed
Summary
This summary is machine-generated.

The CRISPR-Cas13 system precisely targets RNA, advancing RNA regulation studies and RNA-based diagnostics and therapeutics. This review summarizes Cas13 tools, applications, and future development directions.

Keywords:
CRISPR-Cas13RNAcollateral activity

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • The CRISPR-Cas13 system offers precise engineering of endogenous RNAs.
  • This technology significantly advances RNA regulation understanding.
  • It is crucial for developing RNA-based diagnostics and therapeutics.

Purpose of the Study:

  • To summarize Cas13-based RNA targeting tools and applications.
  • To discuss limitations and challenges of current Cas13 tools.
  • To suggest future development directions for RNA targeting systems.

Main Methods:

  • Literature review of CRISPR-Cas13 systems.
  • Analysis of current RNA targeting tools and their applications.
  • Identification of challenges and future research avenues.

Main Results:

  • Comprehensive overview of diverse Cas13 RNA targeting tools.
  • Detailed examination of applications in research and medicine.
  • Identification of key limitations including off-target effects and delivery challenges.

Conclusions:

  • CRISPR-Cas13 is a powerful platform for RNA manipulation.
  • Further development is needed to overcome existing challenges.
  • Future research should focus on enhancing specificity, delivery, and expanding applications.