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Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Surface-Based Multimeric Aptamer Generation and Bio-Functionalization for Electrochemical Biosensing Applications.

Seyed Vahid Hamidi1, Arash Khorrami Jahromi1, Imman I Hosseini1

  • 1Department of Bioengineering, McGill University, Montreal, Quebec, H3A 0E9, Canada.

Angewandte Chemie (International Ed. in English)
|May 20, 2024
PubMed
Summary
This summary is machine-generated.

Multimeric aptamers generated via surface rolling circle amplification (RCA) significantly enhance biosensor sensitivity and affinity. This novel approach offers a 10-fold increase in affinity and a 4-fold increase in sensitivity for detecting targets like SARS-CoV-2 spike protein.

Keywords:
Electrochemical aptasensorMultimeric aptamersNano-/microislandsRoom temperature rolling circle amplification

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Biosensor Engineering

Background:

  • Multimeric aptamers offer superior binding affinity compared to monomeric forms due to multiple binding sites.
  • Developing sensitive and selective biosensors remains a key challenge in analyte detection.

Purpose of the Study:

  • To engineer surface-based multimeric aptamers using room temperature rolling circle amplification (RCA).
  • To develop a highly sensitive and selective electrochemical aptasensor for target analyte detection.
  • To investigate the impact of surface structures on aptasensor performance.

Main Methods:

  • Surface-based generation of multimeric aptamers via RCA with chemically modified primers.
  • Hybridization of multimeric aptamers to spacer primers for proximity to sensing surfaces.
  • Characterization of the surface amplification process and optimization of amplification time.
  • Electrochemical detection using both flat and nano-/microisland (NMI) working electrodes (WEs).
  • Testing with SARS-CoV-2 spike protein (SP) and clinical saliva samples.

Main Results:

  • Multimeric aptasensors exhibited over 10-fold higher affinity and nearly 4-fold higher sensitivity than monomeric aptasensors.
  • Nano-/microisland (NMI) WEs significantly enhanced sensitivity in buffer and saliva.
  • A limit of detection (LOD) below 2 fg/mL was achieved with NMIs multimeric aptasensors.
  • The developed aptasensors were successfully validated with patient saliva samples.

Conclusions:

  • Surface-generated multimeric aptamers via RCA provide a powerful strategy for enhancing aptasensor performance.
  • NMI-based electrode structures combined with multimeric aptamers offer superior sensitivity and selectivity for biosensing applications.
  • The developed electrochemical aptasensor demonstrates clinical potential for detecting biomarkers in complex biological media.