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Updated: Jun 24, 2026

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection
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Microfluidic Transfection System and Temperature Strongly Influence the Efficiency of Transient Transfection.

Michaela Dehne1,2, Simon Valentin Neidinger3, Michael Stark3

  • 1Institute of Technical Chemistry, Leibniz University Hannover, Hannover 30167, Germany.

ACS Omega
|May 20, 2024
PubMed
Summary

A novel 3D-printed microfluidic system enhances transient transfection (TTF) efficiency in CHO-K1 cells. Optimal results were achieved at 22°C, correlating transfection efficiency with cell membrane phase transitions.

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