Related Experiment Video
Updated: Jun 26, 2025
07:47
Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
Published on: September 15, 2023
1.5K
Novel miR-490-3p/hnRNPA1-b/PKM2 axis mediates the Warburg effect and proliferation of colon cancer cells via the
Xiang-Hui Wan1,2,3, Guo-Bing Jin2, Qun Yang2
1Jiangxi Medical College, Nanchang University, Nanchang 330029, Jiangxi Province, China.
World Journal of Gastrointestinal Oncology
|May 20, 2024
Summary
The miR-490-3p/hnRNPA1-b/PKM2 axis promotes colon cancer by enhancing the Warburg effect. Targeting this axis with miR-490-3p inhibits cancer cell proliferation, offering a new diagnostic and therapeutic strategy.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Heterogeneous ribonucleoprotein A1 (hnRNPA1) is implicated in colon cancer (CC) progression.
- The specific role of the miR-490-3p/hnRNPA1-b/PKM2 axis in CC remains unclear.
Purpose of the Study:
- To elucidate the mechanism of the miR-490-3p/hnRNPA1-b/PKM2 axis in CC.
- To investigate its role in promoting the Warburg effect and CC cell proliferation via the PI3K/AKT pathway.
Main Methods:
- Immunohistochemistry, qRT-PCR, and Western blot to assess hnRNPA1-b expression in 220 CC patients.
- Cell proliferation, cell cycle, and apoptosis assays (CCK-8, EdU, flow cytometry).
- Dual luciferase reporter assay to confirm miR-490-3p targeting of hnRNPA1-b; glucose uptake and lactic acid assays for Warburg effect.
Main Results:
- hnRNPA1-b expression is significantly upregulated in CC tissues and cells, correlating with advanced clinical stage and T classification.
- hnRNPA1-b promotes CC cell proliferation and the Warburg effect through the PI3K/AKT pathway.
- miR-490-3p inhibits CC cell proliferation by targeting hnRNPA1-b, thereby suppressing the Warburg effect.
Conclusions:
- The novel miR-490-3p/hnRNPA1-b/PKM2 axis plays a crucial role in colon cancer progression.
- This axis represents a potential new target for colon cancer diagnosis and therapy.
Keywords:
Alternative splicingColon cancerHeterogeneous ribonucleoprotein A1-bMiR-490-3pWarburg effectMore Related Videos
11:13Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
Published on: June 9, 2023
1.5K
06:51Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
Published on: July 21, 2018
17.9K
Related Concept Videos
01:22PI3K/mTOR/AKT Signaling Pathway
3.5K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.5K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:19Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
01:45Destabilization of Microtubules
2.7K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.7K
02:34Inhibition of Cdk Activity
4.7K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.7K