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Assessment of early hemopoiesis in in vivo diffusion chamber cultures
Many studies have provided evidence that the cells which give rise to colonies containing granulocytes (CFU-DG) share several characteristics with other primitive hemopoietic precursors (CFU-S and CFU-MIX). Cell cycle status, sensitivity to hypotonic lysis, and velocity sedimentation rates are similar. On the other hand, CFU-DG can be distinguished from these precursors by difference in sensitivity to cryopreservation and hyperthermia. Uniqueness of the in vivo diffusion chamber culture system is accentuated by finding humoral activities produced by human HUT 102 and Mo T cell lines and murine BCL1 B cell line which seem to be different from the activities identifiable by CFU-C, BFU-E, and CFU-MIX assays in vitro.
Many studies have provided evidence that the cells which give rise to colonies containing granulocytes (CFU-DG) share several characteristics with other primitive hemopoietic precursors (CFU-S and CFU-MIX). Cell cycle status, sensitivity to hypotonic lysis, and velocity sedimentation rates are similar. On the other hand, CFU-DG can be distinguished from these precursors by difference in sensitivity to cryopreservation and hyperthermia. Uniqueness of the in vivo diffusion chamber culture system is accentuated by finding humoral activities produced by human HUT 102 and Mo T cell lines and murine BCL1 B cell line which seem to be different from the activities identifiable by CFU-C, BFU-E, and CFU-MIX assays in vitro.