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Diosmin protects the testicles from doxorubicin-induced damage by increasing steroidogenesis and suppressing

Mega O Oyovwi1,2, Benneth Ben-Azu3, Edesiri P Tesi4

  • 1Department of Physiology, Adeleke University Ede, Osun State, Nigeria.

International Journal of Biochemistry and Molecular Biology
|May 20, 2024
PubMed
Abstract

Insights

Diosmin, an antioxidant, protects against doxorubicin-induced testicular damage and spermatotoxicity by reducing oxidative stress, inflammation, and apoptosis. This study suggests diosmin as a potential therapeutic agent for mitigating chemotherapy side effects.

Area of Science:

  • Reproductive Toxicology
  • Pharmacology
  • Biochemistry

Background:

  • Doxorubicin (DOX) chemotherapy causes testicular damage and spermatotoxicity via oxidative stress, inflammation, and apoptosis.
  • This study investigates the protective effects of diosmin against DOX-induced testicular injury in rats.

Purpose of the Study:

  • To evaluate the ameliorative impact of diosmin on doxorubicin-mediated spermatotoxicity.
  • To assess diosmin's potential as a protective agent against chemotherapy-induced testicular damage.

Main Methods:

  • Rats were divided into four groups: control, diosmin only, doxorubicin only, and doxorubicin with diosmin.
  • Treatments were administered over 8 weeks, followed by evaluation of sperm quality, hormone levels, biochemical markers, and testicular histology.
  • Key parameters assessed included steroidogenic enzymes, oxidative stress markers, inflammatory markers, and apoptosis.

Main Results:

  • Doxorubicin significantly reduced sperm quality, steroidogenic enzyme activity, and hormone levels (FSH, LH, testosterone).
  • DOX treatment increased testicular oxidative damage, inflammation, and apoptosis, confirmed by histopathological findings.
  • Diosmin administration counteracted DOX-induced testicular injury, restoring architecture, preserving spermatogenesis, and reducing inflammation and apoptosis.

Conclusions:

  • Diosmin exhibits antioxidant, anti-apoptotic, and anti-inflammatory properties.
  • Diosmin demonstrates significant potential as a therapeutic agent to protect testicular tissue from doxorubicin-induced toxicity.
  • Diosmin may offer a protective strategy against the adverse reproductive effects of doxorubicin chemotherapy.
Keywords:
Gonadotoxicityapoptosisdiosmindoxorubicininflammationoxidative stressspermatoxicity

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