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Resolving sulfation PTMs on a plant peptide hormone using nanopore sequencing
Xiuqi Chen1,2, Jasper W van de Sande3,2, Justas Ritmejeris1,2
1Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Delft, the Netherlands.
Biorxiv : the Preprint Server for Biology
|May 20, 2024
Summary
Nanopore sequencing accurately identifies sulfation and phosphorylation on peptide phytohormones. This breakthrough offers a sensitive new tool for studying plant growth and development.
Area of Science:
- Plant biology
- Biochemistry
- Analytical chemistry
Background:
- Post-translational modifications (PTMs) on peptide phytohormones are critical for plant growth and development.
- Tyrosine sulfation is a key PTM, but its detection and localization face significant technical hurdles.
- Accurate measurement of PTMs is essential for understanding plant signaling pathways.
Approach:
- Utilized a nanopore peptide sequencing method for single-molecule PTM detection.
- Translocated phytosulfokine (PSK) variants with different PTMs through a nanopore.
- Developed a method to distinguish sulfation from phosphorylation on tyrosine residues.
Key Points:
- Accurately identified sulfation and phosphorylation on tyrosine residues of the phytosulfokine (PSK) peptide hormone.
- Achieved >90% accuracy in distinguishing sulfation from phosphorylation on the same residue.
- Determined the presence or absence of PTMs on adjacent tyrosine residues with >96% accuracy.
Conclusions:
- Nanopore sequencing demonstrates high sensitivity for detecting and localizing PTMs on peptide hormones.
- This method provides a novel tool for analyzing sulfation on peptide phytohormones.
- The technology holds promise for broader applications in identifying various protein PTMs.
