Stochastic to Deterministic: A straightforward approach to create serially perfusable multiscale capillary beds.

Area of Science:

• Biomedical Engineering
• Vascular Biology
• Tissue Engineering

Background:

• * In vitro tissue models require functional vasculature for accurate physiological representation.
• * Existing methods lack control over hierarchical perfusion and direct mechanistic investigation of vascular processes.

Conclusions:

• * The developed method provides a low-barrier-to-entry approach for generating functional vascular networks.
• * This technique facilitates mechanistic studies in vascular biology.
• * The method is readily extendable for use in organoid culture, organ-on-a-chip models, and bioprinted tissues.