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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

4.2K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.2K
Neurulation01:30

Neurulation

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Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the...
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Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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Related Experiment Video

Updated: Jun 26, 2025

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
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Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

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Barcoding Notch signaling in the developing brain.

Abigail Siniscalco1, Roshan Priyarangana Perera1, Jessie E Greenslade1

  • 1Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA.

Biorxiv : the Preprint Server for Biology
|May 20, 2024
PubMed
Summary
This summary is machine-generated.

SABER-seq is a new CRISPR-Cas tool that permanently records transient developmental signals, like Notch signaling, in cell genomes. This enables high-resolution mapping of cell fate determination during development.

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Area of Science:

  • Developmental Biology
  • Genomics
  • Neuroscience

Background:

  • Developmental signaling pathways are crucial for cell fate determination and behavior.
  • Traditional reporters like fluorescent proteins have limitations in scalability and cell-type resolution.
  • A need exists for precise methods to track dynamic signaling events in developing tissues.

Approach:

  • We developed SABER-seq, a CRISPR-Cas based molecular recorder system.
  • SABER-seq captures transient signaling cues as permanent genomic mutations.
  • This system combines a signaling sensor with a barcode recorder for inducible Notch signaling tracking.

Key Points:

  • SABER-seq was applied to map Notch signaling in developing zebrafish brains.
  • The system allows deconstruction of signaling history via single-cell transcriptomics.
  • Identified cell types influenced by Notch signaling and potential differential impacts on terminal fates.

Conclusions:

  • SABER-seq provides a novel platform for scalable, high-resolution mapping of developmental signaling.
  • This technology enhances our understanding of cell fate decisions driven by signaling pathways.
  • Enables detailed analysis of signaling dynamics in complex biological systems.