Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bone Structure01:55

Bone Structure

Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of osseous tissue: cortical and cancellous.
Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Kaempferol Attenuates Spaceflight-Associated Knee Cartilage Degradation by Targeting NOX4-Mediated Mitochondrial Dysfunction.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Pilot spatial transcriptomics of dental pulpitis suggests immune-fibroblast profiling linked to reversibility.

Journal of translational medicine·2026
Same author

The Role of AXL Signaling and Mutant <i>Isocitrate Dehydrogenase 1/2</i> in Conventional Chondrosarcoma.

Cancers·2026
Same author

A novel mineral-organic bone adhesive promotes calvarial bone regeneration in microgravity conditions.

NPJ microgravity·2026
Same author

Bone biology in aging periodontal and peri-implant tissues.

Periodontology 2000·2026
Same author

Using Spatial Transcriptomics to Identify a Diagnostic Molecular Signature Associated with Reversible Dental Pulpitis.

Research square·2026

Related Experiment Video

Updated: Jun 30, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.1K

Methods to Enable Spatial Transcriptomics of Bone Tissues.

Luigi Mancinelli1, Karen Elizabeth Schoedel2, Kurt Richard Weiss3

  • 1Department of Periodontics and Preventive Dentistry, University of Pittsburgh School of Dental Medicine; Center for Craniofacial Regeneration, University of Pittsburgh School of Dental Medicine; lum39@pitt.edu.

Journal of Visualized Experiments : Jove
|May 20, 2024
PubMed
Summary
This summary is machine-generated.

A new method enables spatial transcriptomics on bone tissue, preserving RNA and morphology. This technique is crucial for understanding bone development and diseases like osteosarcoma.

More Related Videos

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K
Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
07:43

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Published on: May 3, 2024

2.7K

Related Experiment Videos

Last Updated: Jun 30, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

7.1K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K
Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
07:43

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Published on: May 3, 2024

2.7K

Area of Science:

  • Molecular Biology
  • Genomics
  • Histology

Background:

  • Spatial transcriptomics analyzes gene expression within tissue context.
  • Analyzing hard tissues like bone with spatial transcriptomics is challenging due to RNA and morphology preservation issues.
  • Understanding cell-tissue relationships is key for development and disease pathology.

Purpose of the Study:

  • To develop a method for generating high-quality spatial transcriptomics data from bone tissue.
  • To overcome challenges in RNA and morphology preservation during hard tissue processing.
  • To provide guidelines for both fresh and archived bone samples.

Main Methods:

  • A novel decalcification method for fresh bone samples to preserve RNA and morphology.
  • Guidelines for processing previously paraffin-embedded bone samples without demineralization.
  • Application of the method to generate spatial transcriptomics data from osteosarcoma bone samples.

Main Results:

  • Successful generation of spatial transcriptomics data from bone tissue.
  • Preservation of both RNA integrity and tissue morphology.
  • Demonstration of the method's utility on bone osteosarcoma tissue bank samples.

Conclusions:

  • The described method effectively enables spatial transcriptomics analysis in bone.
  • This advancement facilitates the study of cellular gene expression in the context of bone biology and disease.
  • The protocol is applicable to both fresh and archived bone samples, expanding research possibilities.