Proteomics analysis of coronary atherosclerotic heart disease with different Traditional Chinese Medicine syndrome...

Area of Science:

• Biochemistry
• Proteomics
• Traditional Chinese Medicine (TCM)

Background:

• Coronary artery disease (CAD) is complex, with Traditional Chinese Medicine (TCM) identifying distinct subtypes like "Qi stagnation and blood stasis syndrome" (QS) and "Qi deficiency and blood stasis syndrome" (QD).
• Percutaneous coronary intervention (PCI) is a common treatment for CAD, but understanding its molecular mechanisms in different TCM-defined subtypes is crucial.

Purpose of the Study:

• To investigate the underlying protein molecular mechanisms of QS and QD subtypes of CAD following PCI.
• To elucidate the intervention mechanism of PCI in treating CAD characterized by these two distinct TCM syndromes.

Main Methods:

• A cohort of 227 QS and 211 QD CAD patients undergoing PCI were recruited.
• Label-free quantitative proteomics analyzed serum protein changes before and 6 months after PCI.

Main Results:

• Significant changes in inflammatory markers, lipid profiles, and clinical scores were observed post-PCI in both groups.
• Proteomic analysis revealed 79 and 95 differentially expressed proteins in QS and QD groups, respectively.
• Distinct pathway alterations were identified: cell adhesion in QS and lipid metabolism in QD, with key proteins like apolipoprotein B and complement factors altered in both.

Conclusions:

• This quantitative proteomic study preliminarily identified biomarkers for CAD in different TCM subtypes treated with PCI.
• The findings provide a foundation for understanding protein profiles associated with PCI treatment in various TCM-defined CAD subtypes.