Related Experiment Video
Updated: Aug 4, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
A validated ultra-performance liquid chromatography with tandem mass spectrometry method for the quantification of
Mário Ferreira Conceição Santos1, Giulia Stavrakas Mirada1, Joice Olinda do Couto2
1Center of Exact, Natural and Health Sciences, Federal University of Espírito Santo - UFES, Alto Universitário, Alegre, Espírito Santo, Brazil.
Abstract:
Brazilian green propolis is used in folk medicine because of its various biological properties. The hydroalcoholic extract of Brazilian green propolis is characteristic for possessing several pharmacological properties. Phytochemical investigations have attributed some of these properties to the presence of compounds, which were chosen as analytical markers. This paper reports the development and analytical validation using UPLC-MS/MS in MRM mode. Veratraldehyde was used as an internal standard in qualitative and quantitative analyses of the extracts. Relative standard deviation values obtained for intra-day and inter-day precision were lower than 4%. Of the five parameters for robustness, wavelength detection and flow rate were the critical ones. Limits of detection and quantification ranged from 0.300 to 39.500 ng.mL-1 and from 1.400 to 85.00 ng.mL-1, respectively. The recoveries were between 94.00 and 119.00%, with relative standard deviation values around 5.0%. The developed method is precise, sensitive, and reliable for analysing Brazilian green propolis.
Related Concept Videos
NMR Spectroscopy of Benzene Derivatives
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
Gas Chromatography: Introduction
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.
High-Performance Liquid Chromatography: Types of Detectors
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...

