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Stomatal maturomics: hunting genes regulating guard cell maturation and function formation from single-cell

Yuming Peng1, Yi Liu1, Yifan Wang1

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PubMed
Summary

This study identifies 586 genes crucial for stomatal maturation and function in Arabidopsis. Two genes, MYS1 and MYS2, were found to redundantly regulate guard cell size and aperture, impacting stomatal function.

Keywords:
G2-like transcription factorsHoop rigidityMYS1MYS2Single-cell analysisStomata maturomicsStomatal aperture

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Stomata are vital for plant gas exchange and pathogen defense.
  • Early stomatal development is well-studied, but guard cell maturation remains less understood.
  • Maturomics, the omics analysis of maturation processes, offers a new approach.

Purpose of the Study:

  • To identify genes involved in guard cell maturation and functional stomata formation.
  • To create a comprehensive gene list for studying stomatal development.
  • To validate identified genes through functional analysis.

Main Methods:

  • Integrative analysis of three public single-cell RNA-seq datasets.
  • Identification of genes upregulated during stomatal maturation.
  • Functional characterization of candidate genes MYS1 and MYS2 in Arabidopsis.

Main Results:

  • A list of 586 specifically upregulated genes (sc_586) was identified across all datasets.
  • The sc_586 list is enriched with known regulators of stomatal maturation and function.
  • MYS1 and MYS2 redundantly regulate guard cell size and rigidity, with double mutants exhibiting impaired stomatal aperture control.

Conclusions:

  • The study provides a valuable resource of 586 genes for understanding guard cell maturation.
  • MYS1 and MYS2 play essential, redundant roles in mature guard cell function.
  • This research advances the understanding of stomatal development and regulation.