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Foliar Inoculation of Phyllachora maydis into Corn Induces Infection and Local Spread in Field Environments
José E Solórzano1, Aarav Subbaiah1, Crystal M Floyd1
1University of Minnesota, Department of Plant Pathology, St. Paul, MN 55108.
Plant Disease
|May 20, 2024
Summary
Researchers developed a reliable field inoculation method for corn tar spot, caused by Phyllachora maydis. This breakthrough enables crucial research into managing this significant agricultural disease.
Area of Science:
- Plant Pathology
- Agricultural Science
- Mycology
Background:
- Tar spot, caused by Phyllachora maydis, is a major corn disease in North America.
- Effective field research for tar spot management is limited by a lack of reliable inoculation methods.
Purpose of the Study:
- To develop and validate a field-based inoculation technique for Phyllachora maydis in Zea mays.
- To facilitate future research on corn tar spot disease dynamics and control strategies.
Main Methods:
- Corn leaves were inoculated with P. maydis inoculum stored at -20°C for 10 months.
- Two field experiments were conducted to assess disease development post-inoculation.
Main Results:
- Stromata of P. maydis were visible 19 days post-inoculation.
- Secondary spread of tar spot was observed 39-41 days after initial inoculation.
- Disease was confined to inoculated areas, confirming successful targeted infection.
Conclusions:
- A practical field inoculation method for corn tar spot using stored P. maydis inoculum was successfully demonstrated.
- This method will advance understanding of the corn tar spot pathosystem and support the development of improved management practices.
