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Related Experiment Videos

Surface markers on lymphocytes leaving pig lymph nodes.

R Pabst, R M Binns, S T Licence

    Immunology
    |October 1, 1985
    PubMed
    Summary

    Pig lymphocytes recirculate directly into the bloodstream from lymph nodes, not through lymphatics. This study quantises lymphocyte emigration and identifies B and T lymphocytes as key emigrating cell types.

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    Cell and tissue research·2014

    Area of Science:

    • Immunology
    • Lymphatic System Physiology
    • Cell Biology

    Background:

    • Lymphocyte recirculation is crucial for immune surveillance.
    • The precise exit route of lymphocytes from lymph nodes into the bloodstream is not fully elucidated.
    • Understanding lymphocyte migration is key to immune response modulation.

    Purpose of the Study:

    • To investigate the in vitro emigration of lymphocytes from pig mesenteric lymph nodes.
    • To determine the primary route of lymphocyte exit from lymph nodes.
    • To characterize the lymphocyte subpopulations involved in emigration.

    Main Methods:

    • In vitro perfusion of pig mesenteric lymph nodes at physiological temperature.
    • Continuous collection of venous effluent to quantify lymphocyte release.
    • Microscopic examination of lymph node paracortical venular walls to identify emigration sites.

    Main Results:

    • Lymphocytes were continuously released into the venous effluent during perfusion.
    • Lymphocytes were observed to emigrate directly into the blood vasculature, bypassing efferent lymphatics.
    • The estimated rate of lymphocyte emigration was 6 x 10^7 cells/hr.
    • B lymphocytes and E-rosette forming T lymphocytes were the primary emigrating subpopulations, with a notable increase in T lymphocytes over time.

    Conclusions:

    • Lymphocyte recirculation involves direct emigration from lymph nodes into the bloodstream.
    • Paracortical venules are suggested as the primary sites of lymphocyte emigration.
    • Lymphocyte subpopulations exhibit differential migratory capacities, with B and T cells being the main emigrators.

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