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An Assay for Quantifying Protein-RNA Binding in Bacteria
Published on: June 12, 2019
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Single-cell transcriptome revealed dysregulated RNA-binding protein expression patterns and functions in human
Zheng Ren1, Chenyang Li2, Jing Wang1
1Xinjiang Institute of Spinal Surgery, Sixth Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang, China.
Frontiers in Medicine
|May 21, 2024
Summary
RNA-binding proteins (RBPs) show distinct expression patterns in immune cells of ankylosing spondylitis (AS). Their dysregulation is linked to AS development, highlighting RBPs as potential therapeutic targets for this inflammatory disease.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Ankylosing spondylitis (AS) is a chronic inflammatory disease primarily affecting the axial skeleton.
- Immune cell dysregulation plays a critical role in the pathogenesis of AS.
- RNA-binding proteins (RBPs) are crucial regulators of gene expression and cellular function, but their specific roles in AS immunity are not fully understood.
Purpose of the Study:
- To investigate the expression characteristics and regulatory patterns of RBPs in various immune cell types within AS patients.
- To identify key RBPs involved in the pathogenesis and progression of AS.
- To elucidate the potential functional roles of RBPs in AS-related immune responses.
Main Methods:
- Utilized single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing (RNA-seq) data from AS patients and healthy controls (HC).
- Performed correlation analysis to identify differentially expressed RBPs and their associated immune cell populations.
- Analyzed the potential involvement of RBPs in key signaling pathways relevant to AS.
Main Results:
- Significant alterations in immune cell composition were observed in AS patients compared to HC, including increased B cells, dendritic cells, and CD14+ monocytes, and decreased platelets and NK cells.
- Specific RBPs, such as DDX3X and SFPQ in B cells, and EIF2S3 and SRSF7 in CD8+ T cells, were identified as potentially important for immune cell differentiation and function in AS.
- Differentially expressed variable splicing genes (RASGs) were implicated in pathways such as transcriptional regulation, protein phosphorylation, and angiogenesis in AS.
Conclusions:
- RBPs exhibit specific expression profiles and regulatory functions across different immune cell types in AS.
- Aberrant expression and dysregulation of RBPs are strongly associated with the occurrence and development of AS.
- Targeting RBPs may offer a novel therapeutic strategy for managing AS.
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