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Comparative analysis of sampling and detection methods for fungal contamination on common healthcare environment
Bobby G Warren1,2,3, Amanda Graves1,2,3, Aaron Barrett1,2,3
1Duke Center for Antimicrobial Stewardship and Infection Prevention, Durham, NC, USA.
Abstract:
We evaluated sampling and detection methods for fungal contamination on healthcare surface materials, comparing the efficacy of foam sponges, flocked swabs, and Replicate Organism Detection And Counting (RODAC) plates alongside culture-based quantification and quantitative polymerase chain reaction (qPCR). Findings indicate that sponge sampling and qPCR detection performed best, suggesting a foundation for future studies aiming to surveillance practices for fungi.
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