The odor of a nontoxic tetrodotoxin analog, 5,6,11-trideoxytetrodotoxin, is detected by specific olfactory sensory neurons of the green spotted puffers
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View abstract on PubMed
Summary
This summary is machine-generated.Toxic puffers use 5,6,11-trideoxytetrodotoxin (TDT) as a chemoattractant. Specific oval olfactory sensory neurons (OSNs) in green spotted puffers physiologically respond to TDT, confirming its role in pufferfish chemical communication and foraging.
Area Of Science
- Neuroscience
- Chemical Ecology
- Sensory Biology
Background
- Toxic puffers accumulate tetrodotoxin (TTX) for defense, acquired from prey.
- Previous research indicated toxic puffers are attracted to 5,6,11-trideoxytetrodotoxin (TDT), a nontoxic analog.
- TDT was suggested to activate olfactory sensory neurons (OSNs) in green spotted puffers.
Purpose Of The Study
- To determine if individual crypt olfactory sensory neurons (OSNs) physiologically respond to 5,6,11-trideoxytetrodotoxin (TDT).
- To characterize the specific sensory neurons involved in TDT detection in green spotted puffers.
Main Methods
- Electroporation was used to express the calcium indicator GCaMP6s in olfactory sensory neurons (OSNs).
- Calcium imaging was employed to monitor OSN responses to TDT and amino acids (AAs).
- Immunohistochemistry was used to examine the morphology and location of TDT-sensitive OSNs.
Main Results
- A distinct group of oval OSNs in green spotted puffers showed a specific calcium response to TDT.
- These TDT-sensitive OSNs did not respond to amino acids (AAs), common food odor cues.
- The morphology and location of these oval OSNs resemble crypt OSNs, supporting their role in TDT detection.
Conclusions
- 5,6,11-trideoxytetrodotoxin (TDT) is specifically detected by crypt olfactory sensory neurons (OSNs) in the green spotted puffer.
- TDT acts as a chemoattractant, potentially aiding in locating conspecific toxic puffers and TTX-bearing prey.
- This sensory mechanism facilitates effective toxification and predator avoidance in pufferfish.
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