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Microbiome of the Eye

Microbiome of the Eye

The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...

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Updated: Jun 26, 2026

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

Published on: July 5, 2017

Human Conjunctival Transcriptome in Acanthamoeba Keratitis: An Exploratory Study.

Gerami D Seitzman^1,2, Jeremy D Keenan^1,2, Thomas M Lietman^1,2

¹Francis I. Proctor Foundation, University of California, San Francisco, California; and.

|May 21, 2024

Summary

This summary is machine-generated.

This study found distinct gene expression patterns in the conjunctiva of patients with Acanthamoeba keratitis (AK). These differences could help diagnose AK and understand its mechanisms.

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Area of Science:

Ophthalmology
Microbiology
Genomics

Background:

Acanthamoeba keratitis (AK) is a serious eye infection.
Diagnosing AK can be challenging due to low culture sensitivity.
Understanding host-pathogen interactions is crucial for effective treatment.

Purpose of the Study:

To identify differences in the conjunctival transcriptome between patients with AK and those with sterile keratitis.
To explore potential diagnostic biomarkers for AK.

Main Methods:

Compared conjunctival transcriptomes of 9 AK patients and 13 pathogen-free keratitis patients.
Utilized metagenomic RNA sequencing for pathogen identification.
Analyzed differentially expressed genes and their associated biological pathways.

Main Results:

Identified 36 differentially expressed genes between AK and sterile keratitis groups.
Highlighted genes involved in cellular signaling, ion transport, and metabolism.
Noted specific genes like CPS1, SLC35B4, STEAP2, ATP2B2, NMNAT3, and AKAP12 as potentially relevant to AK.

Conclusions:

Conjunctival gene expression changes are detectable in AK patients.
Transcriptional signatures may aid in distinguishing AK from sterile keratitis.
Differentially expressed genes offer insights into AK pathophysiology and potential therapeutic targets.