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lncRNA CDKN2B-AS1 is downregulated in patients with ventricular fibrillation in acute myocardial infarction

Ricardo Pan-Lizcano1, Lucía Núñez1,2, Pablo Piñón3

  • 1Instituto de Investigación Biomédica de A Coruña (INIBIC), Grupo de Investigación en Cardiología, Complexo Hospitalario Universitario de A Coruña (CHUAC-SERGAS), GRINCAR-Universidade da Coruña (UDC), A Coruña, Spain.

Plos One
|May 21, 2024
PubMed

Insights

Ventricular fibrillation during acute myocardial infarction is a major cause of death. This study found significantly lower expression of the long non-coding RNA CDKN2B-AS1 in patients experiencing this event.

Area of Science:

  • Cardiovascular Research
  • Molecular Biology
  • Genetics

Background:

  • Ventricular fibrillation (VF) is a primary cause of mortality in acute myocardial infarction (AMI).
  • The genetic underpinnings of VF in AMI are not fully understood, with the role of long non-coding RNAs (lncRNAs) unexplored.
  • lncRNAs are increasingly recognized for their regulatory roles in cardiovascular diseases.

Purpose of the Study:

  • To investigate the differential expression of ten selected lncRNAs in patients with and without VF during AMI.
  • To identify potential lncRNA biomarkers associated with VF in the context of AMI.

Main Methods:

  • Quantitative analysis of ten lncRNAs (CDKN2B-AS1, KCNQ1OT1, LIPCAR, MALAT1, MIAT, NEAT1, SLC16A1-AS1, lnc-TK2-4:2, TNFRSF14-AS1, UCA1) in patient samples.
  • Statistical analysis with Bonferroni correction to determine significance.
  • In silico analysis to predict protein interactions and potential pathways.

Main Results:

  • The lncRNA CDKN2B-AS1 exhibited significantly lower expression in patients with VF compared to those without VF in AMI (P = 2.514 x 10^-5).
  • In silico analysis identified six potential interacting proteins (AGO3, PLD4, POU4F1, ZNF26, ZNF326, ZNF431) for CDKN2B-AS1, though their cardiac expression and link to VF are not established.
  • Existing literature suggests CDKN2B-AS1's involvement in the miR-181a/SIRT1 pathway.

Conclusions:

  • The lncRNA CDKN2B-AS1 is significantly downregulated in patients experiencing VF during AMI.
  • CDKN2B-AS1 may serve as a potential biomarker for VF in AMI.
  • Further research is warranted to elucidate the functional role of CDKN2B-AS1 and its interacting proteins in VF pathophysiology.

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