[Effect of 24,25-dihydroxyvitamin D3 on precursor cells of osteogenesis in immobilized rats]

The experiments were carried out on Wistar SPF rats that were immobilized for 35 days. By heterotopic marrow cell transplantation under the kidney capsule to the normal rats and by cloning these cells in vitro it was found that osteogenetic potentials were significantly inhibited and the amount of osteogenetic precursor cells was reduced. The addition of 24,25(OH)2D3 vitamin (at a dose of 1.25 micrograms per day) to the animal diet led to the normalization of the above parameters. It is assumed that immobilization-associated osteoporosis develops via, among other mechanisms, inhibition of histogenesis of stromal precursor cells. The beneficial role of vitamin D3 is actually the activation of histogenesis of these cells which results in the recovery of bone remodelling during immobilization.