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Growth phase-dependent ribonucleic acid production dynamics.

Muhammad Shoaib1, Akshaya Murugesan2, Sandhanasamy Devanesan3

  • 1Molecular Signaling Group, Faculty of Medicine and Health Technology, Tampere University and BioMediTech, P.O. Box 553, 33101 Tampere, Finland.

International Journal of Biological Macromolecules
|May 21, 2024
PubMed
Summary
This summary is machine-generated.

The initial transcriptional event (TF) ON/OFF dynamics dictate subsequent transcription (TC) in Escherichia coli. Understanding these ON/OFF states reveals how gene expression noise is controlled.

Keywords:
Live cell trackingON/OFF dynamicsPromoter regionSingle molecule studyTranscription delay

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Area of Science:

  • Molecular Biology
  • Microbiology
  • Systems Biology

Background:

  • Transcriptional events are fundamental to cellular processes and population behavior.
  • The ON/OFF states of transcription control gene expression bursts.
  • Mechanisms governing transcriptional ON/OFF kinetics in *Escherichia coli* during different growth phases are not fully understood.

Purpose of the Study:

  • To investigate the ON/OFF mechanism and kinetics of the first transcriptional (TF) and consecutive transcriptional (TC) events.
  • To elucidate the role of promoter regions (Plac and Plac/ara1) in regulating these transcriptional dynamics.
  • To understand how TF dynamics influence TC and gene expression noise.

Main Methods:

  • Single mRNA detection in live *Escherichia coli* cells.
  • Analysis of transcriptional ON/OFF states for TF and TC.
  • Elongation arrest experiments to validate mRNA noise regulation.

Main Results:

  • TF ON/OFF duration exhibits distinct modes, inversely related to TC ON/OFF.
  • Promoter regions influence ON/OFF dynamics, affecting mRNA production in fast and slow-dividing cells.
  • Delayed TF OFF-time impacts TC dynamics and gene expression noise (Fano factor), with regulatory regions playing a key role in noise modulation.

Conclusions:

  • The dynamics of the first transcriptional event (TF) significantly pre-define the overall transcriptional output and population diversity.
  • The upstream promoter region is critical for controlling transcriptional noise by modulating TF OFF-time.
  • This study provides insights into the fundamental mechanisms of gene expression regulation and variability in bacteria.