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Development of a highly sensitive ampicillin sensor utilizing functionalized aptamers.

Linjiao Ren1, Shilin Ma1, Chenlong Li1

  • 1College of Electrical and Information, Zhengzhou University of Light Industry, Zhengzhou 450002, China. qinzr@tju.edu.cn.

Analytical Methods : Advancing Methods and Applications
|May 22, 2024
PubMed
Summary
This summary is machine-generated.

A new fluorescence aptamer sensor detects trace ampicillin (AMP) antibiotic residues. Optimized nanogold-functionalized sequences provide high sensitivity and selectivity for antibiotic detection in water and milk.

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Trace antibiotic residues pose risks to public health and the environment.
  • Sensitive and simple detection methods for antibiotics like ampicillin are crucial.
  • Nanogold-functionalized nucleic acid sequences offer potential for biosensor development.

Purpose of the Study:

  • To develop a sensitive and simple ampicillin (AMP) sensor for trace antibiotic residue detection.
  • To comprehensively analyze the influencing factors of nanogold-functionalized nucleic acid sequence modification.
  • To construct a highly sensitive fluorescence aptamer sensor for ampicillin detection.

Main Methods:

  • Analysis of nanogold-functionalized nucleic acid sequence modification factors (method, base length, type).
  • Optimization of functionalized sequence (ployA6-aptamer) and experimental conditions (binding time, pH, detection time).
  • Construction of a fluorescence aptamer sensor based on fluorescence resonance energy transfer (FRET).

Main Results:

  • Longer nucleic acid chains and adenine (A) bases showed higher saturation modification levels.
  • The optimized sensor detected ampicillin in the range of 0.18-3.11 ng/mL with a detection limit of 0.04 ng/mL.
  • The sensor demonstrated significant selectivity and high average recovery rates (98.71% in tap water, 91.83% in milk).

Conclusions:

  • The study successfully developed a highly sensitive and simple fluorescence aptamer sensor for ampicillin detection.
  • The findings provide a research basis for designing sensitive fluorescence aptamer sensors and nanogold@DNA composite applications.
  • The developed method is versatile and can be adapted for detecting various antibiotics and small biological molecules by changing the aptamer type.