Vesicular profiles in frog perineural cells preserved by rapid-freezing and freeze-substitution

Vesicular profiles in perineurial cells of frog peripheral nerves were examined in tissues preserved by rapid-freezing or by conventional chemical fixation. Tissues were processed immediately after removal from the animal or after remaining in Ringers for several hours. Vesicular profiles were present in perineurial cells in all experimental groups, demonstrating that they are not an artifact of chemical fixation. However, variations in their morphology correlated with the different preservation techniques.