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A high-throughput sequence determination for one cyclic peptide immobilized on a single bead by the one-pot
Takeshi Kasama1, Kiyoshi Nokihara2
1HiPep Laboratories, Nakatsukasa-Cho 486-46, Kamigyo-Ku, Kyoto, 602-8158, Japan.
Abstract:
One cyclic peptide immobilized on one gel-type bead has been employed for the discovery of both interacting peptides and/or medicinal medium-sized molecules. Although high-throughput characterization of recognized peptides has been a bottleneck, here, we describe direct liberation from beads by a one-pot reaction using 2-nitro-5-thiocyanatobenzoic acid followed by mass spectrometry to realize faster and routine sequencing of the peptide on the beads. This is useful for the investigation of protein-protein interactions as well as discovery of drug candidates.
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