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Updated: Jun 25, 2025
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In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
Published on: December 29, 2021
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Interplay between histone variants and chaperones in plants
Jiabing Wu1, Bing Liu2, Aiwu Dong3
1State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, 200438, PR China; Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, School of Life Sciences, Fudan University, Shanghai, 200438, PR China.
Current Opinion in Plant Biology
|May 22, 2024
Summary
Histone chaperones and variants are key regulators of DNA processes in plants. This review explores their complex interactions, structural basis, and roles in gene transcription and development.
Area of Science:
- Molecular Biology
- Genetics
- Plant Science
Background:
- Histone chaperones and variants are essential for eukaryotic DNA replication, transcription, and repair.
- They modulate chromatin accessibility by controlling nucleosome assembly and disassembly.
- Cofactors aid histone chaperones in targeting specific genomic regions for histone exchange.
Purpose of the Study:
- To review recent advancements in the understanding of histone variants and chaperones in plants.
- To discuss the structural intricacies of chaperone-histone complexes.
- To elucidate the mechanisms by which these complexes regulate gene transcription and plant development.
Main Methods:
- Literature review of recent research on histone chaperones and variants in plants.
- Analysis of structural data for chaperone-histone interactions.
- Synthesis of mechanistic studies on chromatin regulation.
Main Results:
- Histone chaperones and variants exhibit complex interplay in plants.
- Structural studies reveal specific binding interfaces and mechanisms of action.
- Cooperative action regulates gene transcription and influences plant development.
Conclusions:
- The interplay between histone chaperones and variants is critical for plant gene regulation and development.
- Understanding these interactions provides insights into chromatin dynamics.
- Further research into their structural and mechanistic aspects is warranted.
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