Engineering a far-red fluorescent probe for rapid detection of Hg(II) ions in both cells and zebrafish

Abnormal accumulation of mercury ions (Hg²⁺) in organisms can lead to severe central nervous system and other diseases. Therefore, the monitoring and detection of Hg²⁺ are of great significance for human health and environmental safety. Herein, we designed and synthesized a novel far-red to NIR emission fluorescent probe (Rho-Hg) based on rhodamine derivative as the fluorophore and thiospirolactone as the recognition site for turn-on detecting of Hg²⁺ in living cells and zebrafish. The probe Rho-Hg displayed superior sensitivity (detection limit = 17.5 nM), rapid response (<1 min), colorimetric change, high selectivity, and moderate pH stability. Leveraging this probe, we realized the real-time monitoring of Hg²⁺ in real samples, living cells and zebrafish. By fostering zebrafish embryos and larvae in Hg²⁺-containing nutrient solution, we noticed that Hg²⁺ was ingested into the zebrafish liver when zebrafish were grown up to 3 days old, and thus we successfully monitored the accumulation and changes of Hg²⁺ during zebrafish growth and development. Thus, the probe Rho-Hg could be a powerful tool for sensitive and real-time monitoring of Hg²⁺ in living systems.