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Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
Published on: March 13, 2020
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Simultaneous quantification of seven B vitamins from wheat grains using UHPLC-MS/MS
Da Cao1, Liesl Heughebaert2, Laura Boffel2
1Laboratory of Functional Plant Biology, Department of Biology, Faculty of Sciences, Ghent University, Ghent, Belgium.
Food Chemistry
|May 22, 2024
Summary
Accurate quantification of seven B vitamins in wheat using UHPLC-MS/MS is now possible. This new method aids in understanding B vitamin stability and nutritional availability in staple grains.
Area of Science:
- Food Science
- Analytical Chemistry
- Human Nutrition
Background:
- B-group vitamins are essential micronutrients for human health.
- B vitamin deficiency is a prevalent global health concern.
- Accurate assessment of B vitamins in staple foods like wheat is crucial.
Purpose of the Study:
- To develop and validate a method for quantifying seven B vitamins in wheat.
- To assess the stability of B vitamins in wheat under various storage conditions.
Main Methods:
- A multi-enzyme extraction technique was employed.
- Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was utilized for quantification.
- Seven free B vitamins (B1, B2, B3, B5, B6, B7, and B9) were analyzed.
Main Results:
- The developed method demonstrated high accuracy (below 15% bias) and precision (below 20% RSD).
- Recoveries for the quantified B vitamins ranged from 81% to 118%.
- The method was successfully applied to evaluate B vitamin stability in stored wheat grains.
Conclusions:
- A robust UHPLC-MS/MS method enables accurate B vitamin quantification in wheat.
- This methodology provides a foundation for future research on B vitamin bioavailability.
- Understanding B vitamin stability in wheat is vital for food fortification and nutritional guidance.
