Extracellular Matrix-Mimetic Intrinsic Versatile Coating Derived from Marine Adhesive Protein Promotes Diabetic Wound...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Wound Healing Research

Background:

• Diabetic wound healing is challenging due to complex microenvironments.
• Abnormal immune regulation, excessive reactive oxygen species (ROS), and bacterial infections impede healing.
• Existing treatments often fall short in addressing these multifaceted issues.

Purpose of the Study:

• To develop an extracellular matrix (ECM)-mimetic coating for enhanced diabetic wound healing.
• To investigate the antioxidant, antibacterial, and immune regulatory properties of the coating.
• To demonstrate the efficacy of the coating in promoting wound repair in various models.

Main Methods:

• An ECM-mimetic coating was assembled in situ from scallop byssal protein (Sbp9Δ) triggered by Ca2+.
• The coating's properties (antioxidant, antibacterial, immune regulatory) were evaluated in vitro.
• Proof-of-concept studies were conducted on diabetic mice, rabbits, ex vivo human skins, and Staphylococcus aureus-infected diabetic mice.

Main Results:

• The Sbp9Δ coating demonstrated outstanding in vitro antioxidant, antibacterial, and immune regulatory functions.
• The coating significantly promoted wound healing in diabetic animal models and ex vivo human skin.
• Mechanism studies revealed reduced bacterial load, inflammation, ROS, and increased M2 macrophages, angiogenesis, and re-epithelialization.

Conclusions:

• The developed Sbp9Δ coating offers a convenient and effective in situ approach for diabetic wound repair.
• The coating improves the wound microenvironment by addressing key pathological factors.
• This biomimetic strategy holds promise for clinical applications in managing complex wounds.