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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Updated: Jun 25, 2025

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Urinary miRNAs: Technical Updates.

Santhi Raveendran¹, Alia Al Massih¹, Muna Al Hashmi¹

¹Omics Core, Integrated Genomics Services (IGS), Research Department, Sidra Medicine, Doha, Qatar.

Microrna (Shariqah, United Arab Emirates)

|May 23, 2024

Summary

This summary is machine-generated.

Urinary microRNAs (miRNAs) show promise as disease biomarkers. This review details current technologies for reliable miRNA isolation and profiling from urine, addressing key technical challenges for accurate disease detection.

Keywords:

biomarkers.cancer isolation miRNA profiling urine

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Area of Science:

Urology
Molecular Biology
Biochemistry

Background:

Urine is an accessible, non-invasive biological fluid ideal for research.
MicroRNAs (miRNAs) are crucial gene regulators with potential as disease biomarkers.
Dysregulated urinary miRNAs are linked to various diseases, including bladder cancer.

Purpose of the Study:

To review current technologies for urinary miRNA isolation and profiling.
To address technical challenges in studying urinary miRNAs.
To highlight the advantages and limitations of existing methods.

Main Methods:

Review of existing literature on urinary miRNA extraction and profiling technologies.
Analysis of methods for miRNA isolation, quality control, and profiling.
Discussion of technical challenges and advancements in the field.

Main Results:

Several technologies exist for urinary miRNA isolation and profiling.
Each technology presents unique advantages and limitations.
Reproducible methods are crucial for reliable biomarker discovery.

Conclusions:

Standardized and dependable methods are essential for studying urinary miRNAs.
Advancements in technology are improving the reliability of urinary miRNA analysis.
Urinary miRNAs hold significant potential for non-invasive disease diagnostics.