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Updated: Jun 25, 2025

Identifying PD-1/PD-L1 Inhibitors with Surface Plasmon Resonance Technology
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Identifying PD-1/PD-L1 Inhibitors with Surface Plasmon Resonance Technology

Published on: May 2, 2025

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Sulforaphane impaired immune checkpoint blockade therapy through activating ΔNP63α/PD-L1 axis in gastric cancer

Qi Zhang1, Chenying Yang2, Zhijian Ma3

  • 1Department of Public Health, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

Molecular Carcinogenesis
|May 23, 2024
PubMed

Insights

Sulforaphane (SFN) suppresses gastric cancer growth but upregulates PD-L1, potentially hindering anti-PD-L1 therapy. This study reveals SFN

Area of Science:

  • Oncology
  • Cancer Biology
  • Immunotherapy

Background:

  • Sulforaphane (SFN) shows anticancer properties in various cancers, including gastric cancer.
  • The impact of SFN on programmed death-ligand 1 (PD-L1) and its role in gastric cancer immunotherapy remain unclear.

Purpose of the Study:

  • To investigate the effect of SFN on gastric cancer cell growth.
  • To elucidate the mechanism by which SFN regulates PD-L1 expression.
  • To determine the influence of SFN on the efficacy of anti-PD-L1 monoclonal antibody (α-PD-L1 mab) therapy in gastric cancer.

Main Methods:

  • In vitro and in vivo studies of SFN on gastric cancer cells.
  • Analysis of PD-L1 expression regulation by SFN, focusing on ΔNP63α.
  • Assessment of the combined effect of SFN and α-PD-L1 mab on gastric cancer cells.

Main Results:

  • SFN demonstrated suppression of gastric cancer cell proliferation in both experimental settings.
  • SFN was found to increase PD-L1 expression by activating ΔNP63α in gastric cancer cells.
  • The presence of SFN diminished the therapeutic efficacy of α-PD-L1 mab against gastric cancer cells.

Conclusions:

  • SFN exhibits a dual role in gastric cancer, inhibiting tumor growth while potentially compromising immunotherapy.
  • A novel mechanism of PD-L1 regulation by SFN via ΔNP63α activation in gastric cancer is identified.
  • Findings suggest careful consideration of SFN use in combination with PD-L1 blockade therapy for gastric cancer patients.
Keywords:
PD‐L1gastric cancerimmune checkpoint blockadesulforaphaneΔNP63α

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