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Updated: May 2, 2026

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Published on: October 26, 2018

A data-driven single-cell and spatial transcriptomic map of the human prefrontal cortex.

Louise A Huuki-Myers¹, Abby Spangler¹, Nicholas J Eagles¹

¹Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD 21205, USA.

Science (New York, N.Y.)

|May 23, 2024

Summary

This summary is machine-generated.

Spatial transcriptomics reveals novel molecular domains in the human neocortex, moving beyond traditional layers. These domains show distinct cell compositions and link to neuropsychiatric disorder genes.

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Area of Science:

Neuroscience
Genomics
Molecular Biology

Background:

Human neocortex organization traditionally studied via histological layers.
Spatial transcriptomics offers new ways to define molecular domains.
Cytoarchitecture limitations in understanding neocortical organization.

Purpose of the Study:

To create a molecular neuroanatomical atlas of the human dorsolateral prefrontal cortex.
To identify transcriptionally defined spatial domains.
To map neuropsychiatric disorder-associated genes and cell types to these domains.

Main Methods:

Visium spatial gene expression platform for atlas generation.
Integration with single-nucleus RNA-sequencing data.
Analysis of anterior-posterior axis of the human dorsolateral prefrontal cortex.
Utilized PsychENCODE and public data for gene/cell type enrichment mapping.

Main Results:

Identified distinct spatial domains based on gene expression.
Revealed unique cell type compositions and interactions within these domains.
Mapped enrichment of neuropsychiatric disorder-associated genes and cell types to specific spatial domains.

Conclusions:

Spatial transcriptomics provides a powerful approach to molecularly define neocortical organization.
Novel spatial domains offer new insights into neocortical structure and function.
These findings link specific neocortical regions to neuropsychiatric disorder risk.