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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jun 23, 2026

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

Label-Free Interference Imaging of Intracellular Trafficking.

Jin-Sung Park¹, Il-Buem Lee¹, Seok-Cheol Hong^1,2

¹Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science, Seoul 02841, Korea.

Accounts of Chemical Research

|May 23, 2024

Summary

Label-free imaging reveals how cells manage intracellular traffic. New methods like Dynamic Scattering-particle Localization Interference Microscopy (DySLIM) visualize cargo transport and cellular strategies for efficient movement.

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Last Updated: Jun 23, 2026

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

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Published on: July 3, 2015

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

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Published on: December 29, 2017

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Published on: August 8, 2019

Area of Science:

Cellular Biology
Biophysics
Microscopy

Background:

Intracellular cargo trafficking is vital but challenging to study dynamically.
Traditional fluorescence imaging has limitations like photobleaching and inability to visualize unlabeled components.
Understanding cellular traffic requires methods that capture the complete lifecycle and environmental context.

Purpose of the Study:

To explore label-free imaging techniques for studying intracellular traffic.
To develop and apply advanced microscopy methods for visualizing nanoscale cargo dynamics.
To investigate cellular strategies for efficient cargo transport in crowded environments.

Main Methods:

Utilized interferometric scattering (iSCAT) microscopy for label-free visualization of nanoscale structures.
Developed Dynamic Scattering-particle Localization Interference Microscopy (DySLIM) for high-speed, quantitative imaging of cargo transport.
Combined DySLIM with chemical-selective fluorescent methods for synergistic analysis.

Main Results:

Successfully visualized the entire lifespan of nascent adhesions (NAs) and their dynamics in living cells.
Demonstrated that intracellular cargos face significant traffic congestion but employ strategies like collective migration and hitchhiking.
Provided quantitative, biophysical insights into nanoscale logistics within living cells.

Conclusions:

Label-free interference-based imaging, particularly DySLIM, offers unprecedented detail for studying intracellular traffic.
Cells utilize sophisticated strategies, akin to urban traffic management, for efficient cargo transport.
Future research should focus on the functional applications of iSCAT live-cell imaging to understand cellular transport phenomena.