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Related Experiment Video

Updated: Jun 25, 2025

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
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High-throughput enrichment of portal venous circulating tumor cells for highly sensitive diagnosis of CA19-9-negative

Zhixian Zhu1, Yixuan Zhang2, Wenjun Zhang3

  • 1School of Mechanical Engineering and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China.

Biosensors & Bioelectronics
|May 23, 2024
PubMed
Summary
This summary is machine-generated.

A novel microfluidic liquid biopsy effectively detects early pancreatic cancer (PC) by enriching circulating tumor cells (CTCs) from portal blood. This method significantly improves diagnosis accuracy for patients negative for the standard CA19-9 biomarker.

Keywords:
Circulating tumor cellInertial microfluidicsPancreatic cancerPortal venous systemTumor-proximal liquid biopsy

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Area of Science:

  • Oncology
  • Biomedical Engineering
  • Diagnostics

Background:

  • Carbohydrate antigen 19-9 (CA19-9) is a common but limited biomarker for pancreatic cancer (PC) diagnosis.
  • CA19-9 lacks sensitivity and specificity, especially in early stages, and some PC patients show negative results.
  • Accurate detection methods for early-stage PC, particularly in CA19-9-negative individuals, are critically needed.

Purpose of the Study:

  • To develop and validate a high-throughput method for enriching circulating tumor cells (CTCs) from portal venous blood for early PC diagnosis.
  • To improve the diagnostic accuracy of pancreatic cancer, especially in patients with low or undetectable CA19-9 levels.
  • To assess the efficacy of combining portal venous CTC analysis with CA19-9 testing.

Main Methods:

  • Coupling tumor-proximal liquid biopsy with inertial microfluidics for CTC enrichment.
  • Utilizing slanted spiral microfluidic channels for size-based CTC separation via inertial focusing and Dean flow effects.
  • Collecting and analyzing portal venous and peripheral blood samples for CTC enumeration.

Main Results:

  • The microfluidic system achieved size-based enrichment of CTCs.
  • Portal venous blood yielded approximately twice the number of CTCs (21.4 cells/5 mL) compared to peripheral blood (10.9 cells/5 mL).
  • Combining peripheral and portal CTC data with CA19-9 results improved diagnostic accuracy for CA19-9-negative PC patients from 47.1% to 87.1%.

Conclusions:

  • Portal venous CTC-based microfluidic biopsy offers high sensitivity and specificity for diagnosing early-stage PC.
  • This approach is particularly valuable for improving the detection of pancreatic cancer in CA19-9-negative patients.
  • The integrated method represents a significant advancement in liquid biopsy for early cancer detection.