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Updated: Jun 25, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

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Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9

Amy R Eggers1, Kai Chen1, Katarzyna M Soczek2

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.

Cell
|May 23, 2024
PubMed
Summary

Engineered Geobacillus stearothermophilus Cas9 (GeoCas9) with mutations in its wedge domain dramatically enhances genome editing in human cells. This improvement stems from accelerated DNA unwinding, offering a new strategy for improving CRISPR-Cas9 technology.

Keywords:
CRISPR-CasCas9 engineeringDNA unwindingGeoCas9R-loop formationWED domaincryo-EMgenome editingiGeoCas9magnesium

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