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Related Experiment Video

Updated: Jun 17, 2026

Single-Molecule Imaging of Nuclear Transport

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

Single molecule delivery into living cells.

Chalmers C Chau^1,2,3, Christopher M Maffeo^4,5, Aleksei Aksimentiev^4,5

¹School of Molecular and Cellular Biology and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, UK.

Nature Communications

|May 23, 2024

Summary

This summary is machine-generated.

This study introduces a nanoinjection platform for precise macromolecule delivery into cells. The system enables single-molecule resolution, quantitative delivery, and phenotypic analysis, advancing cell biology research.

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Last Updated: Jun 17, 2026

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Area of Science:

Cell Biology
Biotechnology
Nanotechnology

Background:

Controlled delivery of macromolecules into cells is crucial for experimental biology.
Existing methods lack precision in molecule quantity and targeting.

Purpose of the Study:

To develop a novel platform for precise macromolecule delivery into cultured cells.
To achieve single-molecule resolution for quantitative cellular manipulation.

Main Methods:

Utilized a nanopipette as both a scanning ion conductance microscope (SICM) probe and an injection tool.
Employed SICM for precise nanopipette positioning and cell insertion.
Demonstrated quantitative delivery of DNA, proteins, and protein fibrils.

Main Results:

Achieved single-molecule resolution for macromolecule delivery into cell lines and primary cells.
Observed cell phenotypic changes dependent on the delivered molecules.
Showed that intracellular macromolecular crowding enhances detection of delivered molecules.

Conclusions:

The nanoinjection platform offers unprecedented control over macromolecule delivery at the single-molecule level.
This technology enables quantitative studies of molecular interactions within cells.
Cellular environment actively aids in the detection of introduced macromolecules.