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Related Concept Videos

Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

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The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
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  1. Home
  3. Research Domains
  5. Agricultural, Veterinary And Food Sciences
  7. Veterinary Sciences
  9. Veterinary Medicine (excl. Urology)
  11. Multidrug-resistant Escherichia Coli Causing Canine Pyometra And Urinary Tract Infections Are Genetically Related But Distinct From Those Causing Prostatic Abscesses

Multidrug-resistant Escherichia coli causing canine pyometra and urinary tract infections are genetically related but distinct from those causing prostatic abscesses

Parinya Sroithongkham1, Naiyaphat Nittayasut1, Jitrapa Yindee1

  • 1Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.

Scientific Reports
|May 23, 2024

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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
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Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice
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View abstract on PubMed

Summary
This summary is machine-generated.

Extraintestinal pathogenic Escherichia coli (ExPEC) in pets can cause serious infections beyond urinary tract infections (UTIs). Genomic analysis reveals high-risk clones like ST131 are linked to pyometra and bloodstream infections in dogs and cats.

Area of Science:

  • Veterinary Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Uropathogenic Escherichia coli (UPEC) causing urinary tract infections (UTIs) are well-characterized.
  • The genetic basis of non-urinary extraintestinal pathogenic E. coli (ExPEC) in companion animals is poorly understood.
  • Companion animals can harbor ExPEC strains with potential for zoonotic transmission.

Purpose of the Study:

  • To characterize virulence traits and genomic backgrounds of ExPEC from canine and feline infections.
  • To investigate the genetic relatedness of ExPEC from urinary and non-urinary sources in companion animals.
  • To identify antimicrobial resistance patterns and high-risk E. coli clones in pet-associated ExPEC.

Main Methods:

  • Isolation and characterization of 104 E. coli strains from canine pyometra, prostatic abscesses, and bloodstream infections, and feline bloodstream infections.
Keywords:
Escherichia coliDogsMultidrug resistanceProstatic abscesses

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  • Antimicrobial susceptibility testing, including third-generation cephalosporins, fluoroquinolones, and extended-spectrum beta-lactamase (ESBL) production.
  • Whole-genome sequencing of 12 multidrug-resistant (MDR) strains and phylogenetic analysis using core SNPs, cgMLST, and pan-genome comparisons.

    • Main Results:

    • Canine pyometra strains showed a stronger association with UPEC than prostatic abscess strains.
    • 44 isolates were resistant to third-generation cephalosporins and/or fluoroquinolones; 15 were ESBL producers.
    • MDR E. coli strains belonged to international high-risk clones (ST38, ST131, ST617, ST648, ST1193), while prostatic abscess strains belonged to distinct lineages.
    • High-risk ST131 and ST1193 (phylogroup B2) harbored numerous ExPEC virulence genes, predominantly in pyometra and UTI isolates.
    • Hybrid MDR/virulence IncF plasmids with aerobactin genes were common in non-B2 phylogroups.

    Conclusions:

    • Genomic insights reveal non-urinary ExPEC in companion animals, including high-risk global clones, possess potential for invasive infections.
    • The study highlights the genetic diversity and shared lineages of ExPEC between human and animal infections.
    • Understanding ExPEC in pets is crucial for veterinary medicine and public health due to potential zoonotic implications.
    Pyometra
    Virulence