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Related Experiment Video

Updated: Jun 25, 2025

Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
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Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

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Capillary blood self-collection for high-throughput proteomics.

Bassim El-Sabawi1, Shi Huang2, Kahraman Tanriverdi1

  • 1Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Proteomics
|May 24, 2024
PubMed
Summary
This summary is machine-generated.

Home capillary blood self-collection using the Tasso+ device showed variable protein concentration results compared to standard venipuncture. This method is best suited for specific proteins with proven agreement in proteomics studies.

Keywords:
blood proteinsblood specimen collectionproteomicstasso

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Area of Science:

  • Biochemistry
  • Proteomics
  • Biotechnology

Background:

  • High-throughput proteomics enables large-scale protein analysis.
  • Capillary blood self-collection offers a convenient alternative to venipuncture.
  • Assessing the reliability of novel collection methods is crucial for clinical applications.

Purpose of the Study:

  • To compare protein concentrations from capillary blood self-collection (Tasso+ device) with standard venipuncture.
  • To evaluate the technical variability and correlation of Olink proteomics data from Tasso+ samples.
  • To determine the suitability of Tasso+ for large-scale proteomics studies.

Main Methods:

  • Collected paired blood samples from 20 volunteers via venipuncture and Tasso+ capillary self-collection.
  • Stored Tasso+ samples at 2°C-8°C for 24 and 48 hours to simulate home collection and shipping.
  • Analyzed protein concentrations using the Olink 384 Inflammatory Panel and assessed correlation (r) and coefficient of variation (CV).

Main Results:

  • Tasso+ blood collection was successful in 37/40 attempts.
  • Protein concentration correlations and CVs varied between Tasso+ and venipuncture samples.
  • At 24 hours, 14.8% of proteins met criteria (r > 0.5, CV < 0.20); at 48 hours, 29.6% met criteria.
  • Overall, only 11.3% of proteins showed good agreement between Tasso+ (24-48h) and venipuncture.

Conclusions:

  • Protein concentrations from Tasso+ capillary blood self-collection exhibit significant technical variability.
  • The correlation with standard venipuncture is variable, especially after 48 hours of simulated transport.
  • Home capillary blood self-collection is recommended for large-scale proteomics only for select proteins demonstrating high concordance with venipuncture.