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The InBIO Barcoding Initiative Database: DNA barcodes of Orthoptera from Portugal.

Sílvia Pina^1,2,3, Joana Pauperio^1,4, Francisco Barros⁵

¹CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, 4485-661 Vairão, Vila do Conde, Portugal CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, 4485-661 Vairão Vila do Conde Portugal.

Biodiversity Data Journal

|May 24, 2024

Summary

This summary is machine-generated.

The InBIO Barcoding Initiative (IBI) dataset provides DNA barcodes for 119 Portuguese Orthoptera species, expanding knowledge of insect biodiversity. This research introduces new DNA barcode data for 54 taxa, enhancing understanding of Orthoptera distribution in Portugal.

Keywords:

DNA barcode Orthoptera continental Portugal cytochrome c oxidase subunit I (COI)species distributions

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Area of Science:

Biodiversity research
Molecular ecology
Entomology

Background:

The InBIO Barcoding Initiative (IBI) dataset encompasses 420 Orthoptera specimens from Portugal.
Morphological identification confirmed 119 species, representing 77% of known Portuguese Orthoptera.

Purpose of the Study:

To generate and publish DNA barcode data for Portuguese Orthoptera.
To expand the Barcode of Life Data System (BOLD) with new insect genetic information.
To document the distribution and genetic diversity of Orthoptera in Portugal.

Main Methods:

Specimen collection in continental Portugal (2005-2021).
Morphological identification by taxonomic experts.
DNA extraction and sequencing for barcoding.
Data submission to the Barcode of Life Data System (BOLD).

Main Results:

DNA barcodes for 54 taxa are newly available on BOLD.
Sequences clustered into 129 Barcode Index Numbers (BINs), with 35 new to BOLD.
Stenobothrus lineatus is newly recorded in continental Portugal.
The dataset significantly enhances DNA barcode and distribution data for Portuguese Orthoptera.

Conclusions:

This study provides a valuable genetic resource for Portuguese Orthoptera.
The findings contribute to a better understanding of insect biodiversity and taxonomy in the region.
Publicly accessible DNA barcodes facilitate future research and conservation efforts.