Genomic, Phylogenetic and Physiological Characterization of the PAH-Degrading Strain Gordonia polyisoprenivorans 135

Ekaterina Frantsuzova ¹, Alexander Bogun ¹, Olga Kopylova ^1,2

⁰Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Scientific Center for Biological Research of Russian Academy of Sciences" (FRC PSCBR RAS), 142290 Pushchino, Moscow Region, Russia.

Biology +

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May 24, 2024

View abstract on PubMed

Summary

Gordonia polyisoprenivorans 135 uniquely degrades aromatic compounds like naphthalene. Its genome reveals atypical genes for polycyclic aromatic hydrocarbon (PAH) metabolism, offering novel biotechnological potential.

Area Of Science

Microbiology
Genomics
Biotechnology

Background

Aromatic compounds are widespread environmental pollutants.
Microbial degradation offers a sustainable solution for bioremediation.
Gordonia polyisoprenivorans is known for its metabolic versatility.

Purpose Of The Study

To investigate the genetic organization of Gordonia polyisoprenivorans 135.
To explore its potential for degrading aromatic compounds.
To identify novel genes and pathways involved in polycyclic aromatic hydrocarbon (PAH) catabolism.

Main Methods

Whole-genome sequencing of Gordonia polyisoprenivorans 135.
Comparative genomic analysis with the Gordonia polyisoprenivorans pangenome.
Analysis of intergenic regions and the 'panIGRome'.
Experimental determination of naphthalene degradation and salicylate utilization.

Main Results

Gordonia polyisoprenivorans 135 demonstrated significant naphthalene degradation (39.43%) and active growth on salicylate.
Genomic analysis revealed atypical genes potentially involved in PAH catabolism, belonging to the broader Actinobacteria pangenome.
Distinct intergenic regions were identified in strain 135 compared to related strains.

Conclusions

Gordonia polyisoprenivorans 135 is a unique strain with confirmed capabilities for degrading naphthalene and utilizing salicylate.
The identified atypical genes suggest novel metabolic pathways for PAH degradation within this species.
Further research into these genetic features could unlock new biotechnological applications for bioremediation.

Keywords:

Gordonia polyisoprenivorans IGR analysis biodegradation genome analysis pangenome polycyclic aromatic compounds