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Plasma osteocalcin in man.

R A Melick, W Farrugia, K J Quelch

    Australian and New Zealand Journal of Medicine
    |August 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    A new radioimmunoassay can measure human osteocalcin, a bone protein. This assay revealed normal levels in healthy adults, with higher levels in certain bone diseases and pregnancy.

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    Area of Science:

    • Endocrinology
    • Biochemistry
    • Bone Metabolism

    Background:

    • Osteocalcin is a key protein in bone metabolism.
    • Accurate measurement of osteocalcin is crucial for diagnosing bone diseases.

    Purpose of the Study:

    • To develop and validate a radioimmunoassay for human osteocalcin.
    • To establish reference ranges for osteocalcin in healthy adults.
    • To investigate osteocalcin levels in various disease states.

    Main Methods:

    • Development of a radioimmunoassay using a bovine osteocalcin standard.
    • Assay validation using human osteocalcin.
    • Measurement of plasma osteocalcin in 212 healthy subjects.
    • Comparison of osteocalcin levels in patients with chronic renal failure, Paget's disease, primary hyperparathyroidism, and pregnancy.

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    Main Results:

    • The developed radioimmunoassay accurately measures human plasma osteocalcin.
    • Mean osteocalcin level in healthy adults was 14.7 ng/ml (geometric mean 12.2 ng/ml), with no age or sex differences.
    • Elevated osteocalcin levels were observed in chronic renal failure, Paget's disease, and primary hyperparathyroidism.
    • Low osteocalcin levels were found during pregnancy.
    • A positive correlation was noted between osteocalcin and alkaline phosphatase.

    Conclusions:

    • The radioimmunoassay is suitable for measuring intact human osteocalcin.
    • Osteocalcin levels vary significantly in specific bone diseases and pregnancy.
    • This assay provides a valuable tool for assessing bone turnover and diagnosing bone-related disorders.